Anticorps Monoclonal anti-NSF

• Phare
• Validé par KD/KO

NSF Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, porc, poulet, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2B6H6

N° de cat : 68061-1-PBS

Synonymes

2B6H6, EC:3.6.4.6, NEM-sensitive fusion protein, N-ethylmaleimide-sensitive fusion protein, SKD2

---

Galerie de données de validation

• 

  WB analysis using 68061-1-Ig (same clone as 68061-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-12 using 68061-1-Ig (same clone as 68061-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 68061-1-Ig (same clone as 68061-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 68061-1-Ig (same clone as 68061-1-PBS)

  WB result of NSF antibody (68061-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSF transfected HEK-293 cells. This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 68061-1-Ig (same clone as 68061-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68061-1-Ig (NSF antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 68061-1-Ig (same clone as 68061-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NSF antibody (68061-1-Ig, Clone: 2B6H6 ) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

68061-1-PBS cible NSF dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, poulet, rat, souris

• Réactivité: Humain, Lapin, porc, poulet, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NSF Protéine recombinante Ag15422
• Nom complet: N-ethylmaleimide-sensitive factor
• Masse moléculaire calculée: 744 aa, 83 kDa
• Poids moléculaire observé: 70-83 kDa
• Numéro d’acquisition GenBank: BC030613
• Symbole du gène: NSF
• Identification du gène (NCBI): 4905
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

{{ptg:RelatedPrimaryAntibodies}}