Anticorps Monoclonal anti-NSFL1C

NSFL1C Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1F12E12

N° de cat : 68182-1-PBS

Synonymes

1F12E12, NSFL1 cofactor p47, p97 cofactor p47, UBX domain-containing protein 2C, UBXN2C

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Galerie de données de validation

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  WB analysis using 68182-1-Ig (same clone as 68182-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68182-1-Ig (NSFL1C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68182-1-PBS in a different storage buffer formulation.

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  WB analysis using 68182-1-Ig (same clone as 68182-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68182-1-Ig (NSFL1C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68182-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 68182-1-Ig (same clone as 68182-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68182-1-Ig (NSFL1C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68182-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 68182-1-Ig (same clone as 68182-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68182-1-Ig (NSFL1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68182-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 68182-1-Ig (same clone as 68182-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using NSFL1C antibody (68182-1-Ig, Clone: 1F12E12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68182-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68182-1-PBS cible NSFL1C dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NSFL1C Protéine recombinante Ag8040
• Nom complet: NSFL1 (p97) cofactor (p47)
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 41 kDa
• Numéro d’acquisition GenBank: BC002801
• Symbole du gène: NSFL1C
• Identification du gène (NCBI): 55968
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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