Anticorps Recombinant de lapin anti-NSMCE2

NSMCE2 Recombinant Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

242862D8

N° de cat : 85188-4-PBS

Synonymes

EC:2.3.2.-, E3 SUMO-protein transferase NSE2, E3 SUMO-protein ligase NSE2, E3 SUMO protein ligase NSE2, C8orf36

---

Galerie de données de validation

• 

  WB analysis using 85188-4-RR (same clone as 85188-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85188-4-RR (NSMCE2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85188-4-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP01897-2

  Sandwich ELISA standard curve of MP01897-2, Human NSMCE2 Recombinant Matched Antibody Pair - PBS only. 85188-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag4545. 85188-5-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

• 

  Sample test of MP01897-2

  The mean NSMCE2 concentration was determined to be 137.24 ng/mL in HeLa cell extract based on a 1.50 mg/mL extract load, 371.47 ng/mL in Jurkat cell extract based on a 1.90 mg/mL extract load and 481.66 ng/mL in U2OS cell extract based on a 3.50 mg/mL extract load.

• 

  IF Staining of U2OS using 85188-4-RR (same clone as 85188-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NSMCE2 antibody (85188-4-RR, Clone: 242862D8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85188-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 85188-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85188-4-RR against Human NSMCE2 were performed. The affinity constant is below 1 pM.

---

Informations sur le produit

85188-4-PBS cible NSMCE2 dans les applications de WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NSMCE2 Protéine recombinante Ag4545
• Nom complet: non-SMC element 2, MMS21 homolog (S. cerevisiae)
• Masse moléculaire calculée: 247 aa, 28 kDa
• Poids moléculaire observé: 28-32 kDa
• Numéro d’acquisition GenBank: BC032797
• Symbole du gène: NSMCE2
• Identification du gène (NCBI): 286053
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NSMCE2 (E3 SUMO-protein ligase NSE2) has a C-terminal Siz/PIAS-RING (SP-RING) domain with E3 SUMO ligase activity (PMID: 15601841). NSMCE2, an essential SUMO ligase of the SMC5/6 complex suppresses cancer and aging in mice (PMID: 26443207).

{{ptg:RelatedPrimaryAntibodies}}