Anticorps Monoclonal anti-NSUN2

• Phare
• Validé par KD/KO

NSUN2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(6)

CloneNo.

2H5A2

Avis (1)

N° de cat : 66580-1-Ig

Synonymes

hTrm4, MISU, NSUN2, SAKI, TRM4

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Galerie de données de validation

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  WB analysis of COLO 320 using 66580-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66580-1-Ig (NSUN2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66580-1-Ig

  WB result of NSUN2 antibody (66580-1-Ig; 1:40000; room temperature for 1.5 hours) with negative control and NSUN2 overexpressed HepG2 cells.

• 

  IP experiment of HepG2 using 66580-1-Ig

  IP result of anti-NSUN2 (IP:66580-1-Ig, 4ug; Detection:66580-1-Ig 1:4000) with HepG2 cells lysate 1920 ug.

• 

  IHC staining of human oesophagus cancer using 66580-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66580-1-Ig (NSUN2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66580-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66580-1-Ig (NSUN2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66580-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66580-1-Ig (NSUN2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66580-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66580-1-Ig (NSUN2 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules Caco-2, cellules HEK-293, cellules HepG2, cellules Jurkat
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu de cancer de l'œsophage humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

66580-1-Ig cible NSUN2 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NSUN2 Protéine recombinante Ag14791
• Nom complet: NOL1/NOP2/Sun domain family, member 2
• Masse moléculaire calculée: 767 aa, 86 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC001041
• Symbole du gène: NSUN2
• Identification du gène (NCBI): 54888
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; N6-methyladenosine modification of circNSUN2 facilitates cytoplasmic export and stabilizes HMGA2 to promote colorectal liver metastasis.; Authors - Ri-Xin Chen
human,	IF	Acta Neuropathol; RNA methyltransferase NSun2 deficiency promotes neurodegeneration through epitranscriptomic regulation of tau phosphorylation; Authors - Yoon A Kim
human	WB,CoIP	Cell Metab; NSUN2 is a glucose sensor suppressing cGAS/STING to maintain tumorigenesis and immunotherapy resistance; Authors - Tingjin Chen
human	WB	Virol Sin; NSUN2 mediates distinct pathways to regulate enterovirus 71 replication; Authors - Lishi Liu
human	WB	Cell Rep; The m5C methyltransferase NSUN2 promotes progression of acute myeloid leukemia by regulating serine metabolism; Authors - Songyu Li
human	WB	Int Immunopharmacol; Hypoxia-induced NSUN2 promotes the progression of colon cancer by modulating the discrepant cleavage of tRNA-Arg; Authors - Na Luan ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Keerthi (Verified Customer) (07-23-2025)

The antibody works very well at the recommended dilutions and performs excellently in both immunoprecipitation and immunoblotting applications. It shows strong specificity and clean bands with minimal background. We also observed consistent results across different batches, which adds to its reliability. Overall, this antibody is a valuable tool for studying target protein interactions and expression levels in our experiments.

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry, Cell culture, ChIP, Protein purification
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Prostate Cancer cells and tissues

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