Anticorps Monoclonal anti-NSUN2

• Phare
• Validé par KD/KO

NSUN2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2H5A2

N° de cat : 66580-1-PBS

Synonymes

hTrm4, MISU, NSUN2, SAKI, TRM4

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Galerie de données de validation

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  WB analysis of COLO 320 using 66580-1-Ig (same clone as 66580-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66580-1-Ig (NSUN2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66580-1-Ig (same clone as 66580-1-PBS)

  WB result of NSUN2 antibody (66580-1-Ig; 1:40000; room temperature for 1.5 hours) with negative control and NSUN2 overexpressed HepG2 cells. This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 66580-1-Ig (same clone as 66580-1-PBS)

  IP result of anti-NSUN2 (IP:66580-1-Ig, 4ug; Detection:66580-1-Ig 1:4000) with HepG2 cells lysate 1920 ug. This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus cancer using 66580-1-Ig (same clone as 66580-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66580-1-Ig (NSUN2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66580-1-Ig (same clone as 66580-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66580-1-Ig (NSUN2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66580-1-Ig (same clone as 66580-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66580-1-Ig (NSUN2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66580-1-Ig (same clone as 66580-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66580-1-Ig (NSUN2 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66580-1-PBS cible NSUN2 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NSUN2 Protéine recombinante Ag14791
• Nom complet: NOL1/NOP2/Sun domain family, member 2
• Masse moléculaire calculée: 767 aa, 86 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC001041
• Symbole du gène: NSUN2
• Identification du gène (NCBI): 54888
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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