Anticorps Recombinant de lapin anti-NSUN2

NSUN2 Recombinant Antibody for IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230186A6

N° de cat : 82894-1-PBS

Synonymes

230186A6, EC:2.1.1.-, EC:2.1.1.-, EC:2.1.1.203, mRNA cytosine C(5)-methyltransferase, Myc-induced SUN domain-containing protein

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Galerie de données de validation

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  Sandwich ELISA sample test of MP00112-3

  HCT-116 cell lysate was measured. The human NSUN2 concentration of detected samples was determined to be 15,374.1 pg/mL (based on a 3.0 mg/mL extract load).

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  Sandwich ELISA standard curve of MP00112-3

  Sandwich ELISA standard curve of MP00112-3, Human NSUN2 Recombinant Matched Antibody Pair - PBS only. 82894-9-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag14791. 82894-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/ml

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  IF Staining of HepG2 using 82894-1-RR (same clone as 82894-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NSUN2 antibody (82894-1-RR, Clone: 230186A6 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82894-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82894-1-RR (same clone as 82894-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human NSUN2 (82894-1-RR, Clone:230186A6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82894-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82894-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82894-1-RR against Human NSUN2 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

82894-1-PBS cible NSUN2 dans les applications de IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NSUN2 Protéine recombinante Ag14791
• Nom complet: NOL1/NOP2/Sun domain family, member 2
• Masse moléculaire calculée: 767 aa, 86 kDa
• Numéro d’acquisition GenBank: BC001041
• Symbole du gène: NSUN2
• Identification du gène (NCBI): 54888
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NSUN2, also known as SAKI or Misu (Myc-induced SUN-domain-containing protein), is a methyltransferase which catalyses (cytosine-5-)-methylation of tRNA. NSUN2 is direct target gene of c-Myc and may act downstream of Myc to regulate epidermal cell growth and proliferation. NSUN2 is overexpressed in various cancer tissues and may be a valuable target for cancer therapy and a cancer diagnostic marker. Recently a splicing mutation in NSUN2 has been identified as the cause of a Dubowitz-like syndrome, an autosomal recessive disorder.

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