Anticorps Polyclonal de lapin anti-NSUN5

• Phare
• Validé par KD/KO

NSUN5 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 15449-1-AP

Synonymes

NOL1, NOL1 related protein, NOL1R, NSUN5, NSUN5A, p120, WBSCR20, WBSCR20A, Ynl022cL

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Galerie de données de validation

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  WB analysis of HeLa using 15449-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15449-1-AP (NSUN5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15449-1-AP

  WB result of NSUN5 antibody (15449-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSUN5 transfected HeLa cells.

• 

  WB analysis of A549 using 15449-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15449-1-AP (NSUN5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 15449-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15449-1-AP (NSUN5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 15449-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 15449-1-AP (NSUN5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse skeletal muscle using 15449-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15449-1-AP (NSUN5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 15449-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15449-1-AP (NSUN5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 15449-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 15449-1-AP (NSUN5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin cancer using 15449-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 15449-1-AP (NSUN5 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 15449-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15449-1-AP (NSUN5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 15449-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15449-1-AP (NSUN5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, tissu placentaire humain, tissu rénal humain
• Résultats positifs en IHC: tissu de muscle squelettique de souris, tissu de cancer de la peau humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

15449-1-AP cible NSUN5 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NSUN5 Protéine recombinante Ag7711
• Nom complet: NOL1/NOP2/Sun domain family, member 5
• Masse moléculaire calculée: 46.6 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC008084
• Symbole du gène: NSUN5
• Identification du gène (NCBI): 55695
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Nat Chem Biol; Activity-based RNA-modifying enzyme probing reveals DUS3L-mediated dihydrouridylation.; Authors - Wei Dai ; KD Validated
mouse	WB,IF	Glia; Cognitive deficits in mice lacking Nsun5, a cytosine-5 RNA methyltransferase, with impairment of oligodendrocyte precursor cells.; Authors - Tingting Zhang
mouse	IF	Cells; Agenesis and Hypomyelination of Corpus Callosum in Mice Lacking Nsun5, an RNA Methyltransferase.; Authors - Zihao Yuan ; KO Validated
human	IHC	Front Oncol; Identification of Expression Patterns and Potential Prognostic Significance of m5C-Related Regulators in Head and Neck Squamous Cell Carcinoma.; Authors - Zhenyuan Han
mouse	WB,IF	Mol Brain; Expression of the RNA methyltransferase Nsun5 is essential for developing cerebral cortex.; Authors - Peipei Chen ; KO Validated
human	WB	Cell Biochem Biophys; NSUN5-FTH1 Axis Inhibits Ferroptosis to Promote the Growth of Gastric Cancer Cells; Authors - Yibin Su ; KD Validated