Anticorps Recombinant de lapin anti-NSUN5

NSUN5 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

242168D5

N° de cat : 84768-2-RR

Synonymes

NOL1-related protein, NOL1R, NOL1, EC:2.1.1.-, 28S rRNA (cytosine-C(5))-methyltransferase

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Galerie de données de validation

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  WB analysis using 84768-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84768-2-RR (NSUN5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 84768-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NSUN5 antibody (84768-2-RR, Clone: 242168D5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of A431 using 84768-2-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using NSUN5 antibody (84768-2-RR, Clone: 242168D5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84768-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84768-2-RR against Human NSUN5 were performed. The affinity constant is 0.69 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549
• Résultats positifs en IF/ICC: cellules HeLa, cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84768-2-RR cible NSUN5 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NSUN5 Protéine recombinante Ag7711
• Nom complet: NOL1/NOP2/Sun domain family, member 5
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 47~51 kDa
• Numéro d’acquisition GenBank: BC008084
• Symbole du gène: NSUN5
• Identification du gène (NCBI): 55695
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NSUN5 is a conserved RNA methyltransferase belonging to the Nop2/SUN domain family, which is highly conserved from archaea to eukaryotes and has close contact with RNA methylation because of their S-adenosyl methionine binding-domain. NSUN5 was a promoter in the progression of CRC mainly through cell cycle regulation (PMID: 32774740). Loss of NSUN5 induces growth phenotypes in mice and different mammalian cells, which is likely connected to the requirement of NSUN5 for ribosomal RNA modification and normal global protein translation, especially in highly proliferative cells (PMID: 31722427). Western blot analysis detected NSUN5 at an apparent molecular mass of 47 kDa.

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