Anticorps Recombinant de lapin anti-NSUN7

NSUN7 Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242358B7

N° de cat : 84795-1-PBS

Synonymes

242358B7, FLJ14001

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Galerie de données de validation

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  WB analysis using 84795-1-RR (same clone as 84795-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84795-1-RR (NSUN7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84795-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 84795-1-RR (same clone as 84795-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84795-1-RR (NSUN7 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84795-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01594-1

  Cytometric bead array standard curve of MP01594-1, NSUN7 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84795-1-PBS. Detection antibody: 84795-2-PBS. Standard: Ag11650. Range: 0.781-100 ng/mL.

• 

  Affinity and Kinetic Characterization of 84795-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84795-1-RR against Human NSUN7 were performed. The affinity constant is 3.58 nM.

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Informations sur le produit

84795-1-PBS cible NSUN7 dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NSUN7 Protéine recombinante Ag11650
• Nom complet: NOL1/NOP2/Sun domain family, member 7
• Masse moléculaire calculée: 718 aa, 81 kDa
• Poids moléculaire observé: 81~90 kDa
• Numéro d’acquisition GenBank: BC036568
• Symbole du gène: NSUN7
• Identification du gène (NCBI): 79730
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NSUN7 (NOP2/Sun RNA methyltransferase family member 7) is involved in mitochondrial rRNA processing in post-meiotic sperm and is predicted to act upstream of or within flagellated sperm motility and sperm mitochondrion organization. In humans, Nsun7 encodes a protein with 718-amino acid and 3697-bp belonging to the methyltransferase superfamily (PMID: 37173708). The failure to produce NSUN7 would lead to the impairment in activity and motility of sperm flagella, leading to sperm motility deficit and infertility (PMID: 25702163).

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