Anticorps Polyclonal de lapin anti-NT5DC1

NT5DC1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 24102-1-PBS

Synonymes

NT5C2L1, EC:3.1.3.-, 5'-nucleotidase domain-containing protein 1

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Galerie de données de validation

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  WB analysis of MCF-7 using 24102-1-AP (same clone as 24102-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 24102-1-AP (NT5DC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24102-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 24102-1-AP (same clone as 24102-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24102-1-AP (NT5DC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24102-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 24102-1-AP (same clone as 24102-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24102-1-AP (NT5DC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24102-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 24102-1-AP (same clone as 24102-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24102-1-AP (NT5DC1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24102-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 24102-1-AP (same clone as 24102-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24102-1-AP (NT5DC1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24102-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 24102-1-AP (same clone as 24102-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 24102-1-AP (NT5DC1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24102-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 24102-1-AP (same clone as 24102-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 24102-1-AP (NT5DC1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24102-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 24102-1-AP (same clone as 24102-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NT5DC1 antibody (24102-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 24102-1-PBS in a different storage buffer formulation.

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Informations sur le produit

24102-1-PBS cible NT5DC1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NT5DC1 Protéine recombinante Ag20916
• Nom complet: 5'-nucleotidase domain containing 1
• Masse moléculaire calculée: 455 aa, 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC015138
• Symbole du gène: NT5DC1
• Identification du gène (NCBI): 221294
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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