Anticorps Polyclonal de lapin anti-NT5E/CD73

• Phare
• Validé par KD/KO

NT5E/CD73 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

40

Avis (2)

N° de cat : 12231-1-AP

Synonymes

5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), CD 73, CD73, E5NT, Ecto 5 nucleotidase, ecto-5'-NT, Ecto-5'-nucleotidase, eN, eNT, NT, NT5, NT5E, NT5E/CD73, NTE

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Galerie de données de validation

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  WB analysis using 12231-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12231-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12231-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 12231-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 12231-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 12231-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12231-1-AP (NT5E/CD73 antibody) at dilution of 1:1000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse small intestine using 12231-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using NT5E/CD73 antibody (12231-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse small intestine using 12231-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using NT5E/CD73 antibody (12231-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of NCCIT using 12231-1-AP

  1X10^6 NCCIT cell were stained with 0.2ug NT5E/CD73 antibody (12231-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules A375, cellules HT-29, cellules HuH-7, cellules Jurkat, cellules K-562, tissu cérébral de souris, tissu splénique humain
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer de l'estomac humain, tissu de cancer du côlon humain, tissu de côlon humain, tissu d'estomac humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu d'intestin grêle de souris,
• Résultats positifs en cytométrie: cellule NCCIT

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 20 publications below
• IHC: See 14 publications below
• IF: See 13 publications below
• ELISA: See 1 publications below
• FC: See 5 publications below

Informations sur le produit

12231-1-AP cible NT5E/CD73 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NT5E/CD73 Protéine recombinante Ag2869
• Nom complet: 5'-nucleotidase, ecto (CD73)
• Masse moléculaire calculée: 29 kDa, 63 kDa
• Poids moléculaire observé: 63-70 kDa
• Numéro d’acquisition GenBank: BC015940
• Symbole du gène: NT5E
• Identification du gène (NCBI): 4907
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NT5E(5'-nucleotidase) is also named as NT5, NTE,CD73,ecto-5'-nucleotidase, and belongs to the 5'-nucleotidase family. It catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP.The enzyme occurs mainly as a dimer and the wide distribution of NT5E in the rat hippocampus, suggesting their involvement in the control of the purinergic signaling(PMID:17619139). This protein has two isoforms with the molecular weight of 63 kDa and 58 kDa and four glycosylation sites with a signalpeptide. It can exsit as a dimer(PMID:17619139). It can be detected the band of 55 kDa,64 kDa, 70 kDa, 110 kDa by western blot(PMID:17619139; 12370277; 21533188). Defects in NT5E are the cause of calcification of joints and arteries (CALJA).

Publications

Species	Application	Title
human,mouse	IHC,WB,IF	Int J Biol Sci; CD73/NT5E-mediated ubiquitination of AURKA regulates alcohol-related liver fibrosis via modulating hepatic stellate cell senescence; Authors - Zhenni Liu
human	WB,IHC	Br J Cancer; Norepinephrine inhibits CD8+ T-cell infiltration and function, inducing anti-PD-1 mAb resistance in lung adenocarcinoma; Authors - Qishun Geng
mouse	IF	Theranostics; GATA4-driven miR-206-3p signatures control orofacial bone development by regulating osteogenic and osteoclastic activity.; Authors - Shuyu Guo
	IHC	Theranostics; A sericin/ graphene oxide composite scaffold as a biomimetic extracellular matrix for structural and functional repair of calvarial bone.; Authors - Chao Qi
human	WB	Biochim Biophys Acta Mol Basis Dis; Extracellular ATP (eATP) inhibits the progression of endometriosis and enhances the immune function of macrophages; Authors - Ling Zhou
rat	FC	J Cell Physiol; Mesenchymal stem cells overexpressing heme oxygenase-1 ameliorate lipopolysaccharide-induced acute lung injury in rats.; Authors - Xuxin Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Alessandro (Verified Customer) (12-09-2023)

good product for ICC

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: ROs

FH

Neta (Verified Customer) (07-28-2022)

staining did not work

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mouse small intestine, fixed frozen