Anticorps Recombinant de lapin anti-NT5E

NT5E Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251250C6

N° de cat : 86512-2-PBS

Synonymes

CD73, 5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), 5'-deoxynucleotidase

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Galerie de données de validation

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  Sandwich ELISA sample test of MP02481-1

  The mean CD73 concentration was determined to be 67.84 ng/mL in U-87 MG cell extract based on a 1.5 mg/mL extract load and 125.52 ng/mL in PC-3 cell extract based on a 1.1 mg/mL extract load.

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  Sandwich ELISA sample test of MP02481-1

  Plasma of six individual healthy human donors was measured. The human CD73 concentration of detected samples was determined to be 15.14 ng/mL with a range of 8.55 - 28.73 ng/mL.

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  Sandwich ELISA standard curve of MP02481-1

  Sandwich ELISA standard curve of MP02481-1, Human CD73 Recombinant Matched Antibody Pair - PBS only. 86512-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2974. 86512-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

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  Cytometric bead array sample test of MP02481-1

  The mean CD73 concentration was determined to be 895.3 ng/mL in U-87 MG cell extract based on a 1.5 mg/mL extract load.

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  Cytometric bead array standard curve of MP02481-1

  Cytometric bead array standard curve of MP02481-1, CD73 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86512-2-PBS. Detection antibody: 86512-1-PBS. Standard: Eg2974. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 86512-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 86512-2-PBS against Human NT5E was performed. The affinity constant is 1.45 nM.

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Informations sur le produit

86512-2-PBS cible NT5E dans les applications de Cytometric bead array, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NT5E Protéine recombinante Eg2974
• Nom complet: 5'-nucleotidase, ecto (CD73)
• Masse moléculaire calculée: 63 kDa
• Numéro d’acquisition GenBank: NM_002526.3
• Symbole du gène: CD73
• Identification du gène (NCBI): 4907
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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