Anticorps Monoclonal anti-NT5E,CD73

• Phare
• Validé par KD/KO

NT5E,CD73 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

1G5G8

N° de cat : 67789-1-Ig

Synonymes

5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), CD73, E5NT, Ecto 5 nucleotidase, eN, eNT, NT, NT5, NT5E, NT5E,CD73, NTE

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Galerie de données de validation

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  WB analysis of HepG2 using 67789-1-Ig

  WB result of NT5E,CD73 antibody (67789-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NT5E,CD73 transfected HepG2 cells.

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  WB analysis of A549 using 67789-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 67789-1-Ig (NT5E,CD73 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-29 using 67789-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67789-1-Ig (NT5E,CD73 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-87 MG using 67789-1-Ig

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 67789-1-Ig (NT5E,CD73 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 67789-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67789-1-Ig (NT5E,CD73 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 67789-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67789-1-Ig (NT5E,CD73 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 67789-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67789-1-Ig (NT5E,CD73 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 67789-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67789-1-Ig (NT5E,CD73 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat liver using 67789-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using NT5E,CD73 antibody (67789-1-Ig, Clone: 1G5G8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of rat liver using 67789-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using NT5E,CD73 antibody (67789-1-Ig, Clone: 1G5G8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 67789-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using NT5E,CD73 antibody (67789-1-Ig, Clone: 1G5G8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HepG2, cellules HT-29, cellules MG U-87
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu rénal de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu d'amygdalite humain, tissu hépatique de rat

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67789-1-Ig cible NT5E,CD73 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NT5E,CD73 Protéine recombinante Ag30616
• Nom complet: 5'-nucleotidase, ecto (CD73)
• Masse moléculaire calculée: 29 kDa, 63 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC015940
• Symbole du gène: NT5E
• Identification du gène (NCBI): 4907
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).