Anticorps Monoclonal anti-NTF2

• Phare
• Validé par KD/KO

NTF2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4H7F9

N° de cat : 66063-1-PBS

Synonymes

NTF 2, NTF2, nuclear transport factor 2, NUTF 2, NUTF2, Placental protein 15, PP15

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Galerie de données de validation

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  WB analysis of HeLa using 66063-1-Ig (same clone as 66063-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66063-1-Ig (NTF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66063-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66063-1-Ig (same clone as 66063-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66063-1-Ig (NTF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66063-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 66063-1-Ig (same clone as 66063-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66063-1-Ig (NTF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66063-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66063-1-Ig (same clone as 66063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66063-1-Ig (NTF2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66063-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66063-1-Ig (same clone as 66063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66063-1-Ig (NTF2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66063-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66063-1-Ig (same clone as 66063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66063-1-Ig (NTF2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66063-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66063-1-Ig (same clone as 66063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66063-1-Ig (NTF2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66063-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66063-1-Ig (same clone as 66063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66063-1-Ig (NTF2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66063-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66063-1-Ig (same clone as 66063-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66063-1-Ig (NTF2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66063-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66063-1-PBS cible NTF2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NTF2 Protéine recombinante Ag7879
• Nom complet: nuclear transport factor 2
• Masse moléculaire calculée: 14 kDa
• Poids moléculaire observé: 13 kDa
• Numéro d’acquisition GenBank: BC002348
• Symbole du gène: NTF2
• Identification du gène (NCBI): 10204
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NUTF2 (nuclear transport factor 2), also named as NTF2, is a cytosolic protein responsible for nuclear import of Ran, a small Ras-like GTPase involved in a number of critical cellular processes, including cell cycle regulation, chromatin organization during mitosis, reformation of the nuclear envelope following mitosis, and controlling the directionality of nucleocytoplasmic transport. Nucleocytoplasmic translocation of NTF2 is regulated in mammalian cells, and may involve a tyrosine and/or threonine kinase-dependent signal transduction mechanism(s) (PMID: 22880006). The MW of this protein is 14 kDa, and this antibody specially recognises the 14 kDa protein.

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