Anticorps Polyclonal de lapin anti-Neurotrophin 3

Neurotrophin 3 Polyclonal Antibody for IF, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 18084-1-AP

Synonymes

HDNF, Nerve growth factor 2, Neurotrophic factor, neurotrophin 3, NGF 2, NGF2, NT 3, NT3, NTF3

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Galerie de données de validation

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  WB analysis of SH-SY5Y using 18084-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18084-1-AP (Neurotrophin 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 18084-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18084-1-AP (Neurotrophin 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 18084-1-AP

  IP Result of anti-Neurotrophin 3 (IP:18084-1-AP, 4ug; Detection:18084-1-AP 1:800) with SH-SY5Y cells lysate 1600ug.

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  IF Staining of SH-SY5Y using 18084-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 18084-1-AP (Neurotrophin 3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, tissu placentaire humain
• Résultats positifs en IP: cellules SH-SY5Y
• Résultats positifs en IF: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

18084-1-AP cible Neurotrophin 3 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Neurotrophin 3 Protéine recombinante Ag12798
• Nom complet: neurotrophin 3
• Masse moléculaire calculée: 257 aa, 29 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC069773
• Symbole du gène: NTF3
• Identification du gène (NCBI): 4908
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IHC	ACS Appl Mater Interfaces; Surface Coating of Pulmonary siRNA Delivery Vectors Enabling Mucus Penetration, Cell Targeting, and Intracellular Radical Scavenging for Enhanced Acute Lung Injury Therapy.; Authors - Jiaojiao Zhu
human	WB	Stem Cell Res Ther; NT3P75-2 gene-modified bone mesenchymal stem cells improve neurological function recovery in mouse TBI model.; Authors - Ke Wu
	WB	Front Pharmacol; Synergic Neuroprotection Between Ligusticum Chuanxiong Hort and Borneol Against Ischemic Stroke by Neurogenesis via Modulating Reactive Astrogliosis and Maintaining the Blood-Brain Barrier.; Authors - Bin Yu
mouse	WB	Brain Res Bull; PPARα contributes to the therapeutic effect of hydrogen gas against sepsis-associated encephalopathy with the regulation to the CREB-BDNF signaling pathway and hippocampal neuron plasticity-related gene expression.; Authors - Yuanyuan Bai
rat	WB,IHC	Neurochem Res; Human Umbilical Cord Mesenchymal Stem Cells Overexpressing Nerve Growth Factor Ameliorate Diabetic Cystopathy in Rats.; Authors - Wu WenBo
mouse	IF	Neurosci Lett; Matrine promotes NT3 expression in CNS cells in experimental autoimmune encephalomyelitis.; Authors - Ming-Liang Zhang