Anticorps Polyclonal de lapin anti-NTHL1

NTHL1 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 14918-1-AP

Synonymes

NTH1, NTHL1, OCTS3

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Galerie de données de validation

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  WB analysis using 14918-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14918-1-AP (NTHL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 14918-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 14918-1-AP (NTHL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Y79 using 14918-1-AP

  IP result of anti-NTHL1 (IP:14918-1-AP, 4ug; Detection:14918-1-AP 1:500) with Y79 cells lysate 2000ug.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules K-562, cellules Y79
• Résultats positifs en IP: cellules Y79

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

14918-1-AP cible NTHL1 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NTHL1 Protéine recombinante Ag6734
• Nom complet: nth endonuclease III-like 1 (E. coli)
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC000391
• Symbole du gène: NTHL1
• Identification du gène (NCBI): 4913
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NTHL1, also named as Endonuclease III-like protein 1 or OCTS3, is a 312 amino acid protein, which belongs to the Nth/MutY family. NTHL1 localizes in the nucleus and is widely expressed with highest levels in heart and lowest levels in lung and liver. NTHL1 Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity and incises damaged DNA at cytosines, thymines and guanines. NTHL1 acts on a damaged strand, 5' from the damaged site and is required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions.

Publications

Species	Application	Title
human	WB	Nature; Breakage of cytoplasmic chromosomes by pathological DNA base excision repair.; Authors - Shangming Tang
human	WB	Nat Commun; Mitochondrial respiratory function is preserved under cysteine starvation via glutathione catabolism in NSCLC; Authors - Nathan P Ward

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