Anticorps Polyclonal de lapin anti-NUCKS1

• Phare
• Validé par KD/KO

NUCKS1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12023-2-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis using 12023-2-AP (same clone as 12023-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12023-2-AP (NUCKS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12023-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 12023-2-AP (same clone as 12023-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12023-2-AP (NUCKS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12023-2-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 12023-2-AP (same clone as 12023-2-PBS)

  IP result of anti-NUCKS1 (IP:12023-2-AP, 4ug; Detection:12023-2-AP 1:600) with HEK-293 cells lysate 960 ug. This data was developed using the same antibody clone with 12023-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 12023-2-AP (same clone as 12023-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12023-2-AP (NUCKS1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12023-2-PBS in a different storage buffer formulation.

• 

  IHC staining of rat pancreas using 12023-2-AP (same clone as 12023-2-PBS)

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 12023-2-AP (NUCKS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12023-2-PBS in a different storage buffer formulation.

• 

  IHC staining of rat pancreas using 12023-2-AP (same clone as 12023-2-PBS)

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 12023-2-AP (NUCKS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12023-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 12023-2-AP (same clone as 12023-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12023-2-AP (NUCKS1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12023-2-PBS in a different storage buffer formulation.

---

Informations sur le produit

12023-2-PBS cible NUCKS1 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NUCKS1 Protéine recombinante Ag2652
• Nom complet: nuclear casein kinase and cyclin-dependent kinase substrate 1
• Masse moléculaire calculée: 243 aa, 27 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC000805
• Symbole du gène: NUCKS1
• Identification du gène (NCBI): 64710
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Nuclear ubiquitous casein and cyclin-dependent kinase substrate 1 (NUCKS1) is a nuclear protein that is highly conserved in vertebrates. The conserved regions of the protein contain several consensus phosphorylation sites for casein kinase II and cyclin-dependent kinases, two putative nuclear localization signals, and a basic DNA-binding domain. It is phosphorylated by CDK1 and casein kinase during mitosis of the cell cycle. Phosphorylated upon DNA damage, probably by ATM or ATR. Widelyexpressed, with highest levels in thyroid gland, prostate and uterus and in fetal liver, thymus and lung. Two isoforms of NUCKS1 exist due to alternative splicing events.

{{ptg:RelatedPrimaryAntibodies}}