Anticorps Polyclonal de lapin anti-NUDT12

NUDT12 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

Avis (3)

N° de cat : 17487-1-AP

Synonymes

DeNADding enzyme NUDT12, EC:3.6.1.-, EC:3.6.1.22, NAD-capped RNA hydrolase NUDT12, NADH pyrophosphatase NUDT12

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Galerie de données de validation

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  WB analysis using 17487-1-AP

  mouse liver tissues were subjected to SDS PAGE followed by western blot with 17487-1-AP (NUDT12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17487-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17487-1-AP (NUDT12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 17487-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17487-1-AP (NUDT12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 17487-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 17487-1-AP (NUDT12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 17487-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 17487-1-AP (NUDT12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 17487-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NUDT12 antibody (17487-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of MCF-7 using 17487-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NUDT12 antibody (17487-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HeLa
• Résultats positifs en IHC: tissu pulmonaire humain, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

17487-1-AP cible NUDT12 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NUDT12 Protéine recombinante Ag11557
• Nom complet: nudix (nucleoside diphosphate linked moiety X)-type motif 12
• Masse moléculaire calculée: 462 aa, 52 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC041099
• Symbole du gène: NUDT12
• Identification du gène (NCBI): 83594
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NUDT12 is a member of the Nudix superfamily of pyrophosphohydrolases that has a broad range of substrates, including (d)NTPs, nucleotides, capped RNAs, dinucleotide coenzymes (NAD, FAD, dpCoA), and their derivatives. NUDT12 is active only as homodimers, with each monomer contributing to creation of the two functional catalytic pockets (PMID: 37301333, 31875550). The NUDT12 gene on chromosome 5q21 encodes the open reading frame of the putative 462-amino-acid, 52 kDa NUDT12 protein (PMID: 12790796).

Publications

Species	Application	Title
human	WB	Cell Death Dis; A tumor-specific modulation of heterogeneous ribonucleoprotein A0 promotes excessive mitosis and growth in colorectal cancer cells.; Authors - Hiroaki Konishi

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aleksandr (Verified Customer) (07-19-2025)

Great antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: RKO

NUDT12 Antibody Western Blot validation (1:2000 dilution) in RKO (Cat no:17487-1-AP)

FH

Tsimafei (Verified Customer) (11-19-2024)

Antibody detect a band with the expected molecular weight of Nudt12

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: U2OS

NUDT12 Antibody Western Blot validation (1:3000 dilution) in U2OS (Cat no:17487-1-AP)

FH

Moritz (Verified Customer) (09-12-2024)

worked well for detecting NUDT12 in WB. band was specific and slightly below 50kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T

NUDT12 Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:17487-1-AP)

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