Anticorps Polyclonal de lapin anti-NUDT6

• Phare
• Validé par KD/KO

NUDT6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 11181-1-AP

Synonymes

gfg, FGF2AS, FGF AS, FGF 2, EC:3.6.1.-

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Galerie de données de validation

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  WB analysis of Jurkat using 11181-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11181-1-AP (NUDT6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11181-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11181-1-AP (NUDT6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 11181-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 11181-1-AP (NUDT6 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 11181-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 11181-1-AP (NUDT6 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11181-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11181-1-AP (NUDT6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 11181-1-AP

  IP result of anti-NUDT6 (IP:11181-1-AP, 4ug; Detection:11181-1-AP 1:500) with HepG2 cells lysate 4000ug.

• 

  IHC staining of human liver using 11181-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11181-1-AP (NUDT6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 11181-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11181-1-AP (NUDT6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11181-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11181-1-AP (NUDT6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11181-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11181-1-AP (NUDT6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 11181-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 11181-1-AP (NUDT6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 11181-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 11181-1-AP (NUDT6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11181-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NUDT6 antibody (11181-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HepG2, tissu cardiaque de rat, tissu cardiaque humain, tissu hépatique de rat
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu hépatique humain, tissu cérébral de souris, tissu de côlon de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

11181-1-AP cible NUDT6 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NUDT6 Protéine recombinante Ag1638
• Nom complet: nudix (nucleoside diphosphate linked moiety X)-type motif 6
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 72 kDa
• Numéro d’acquisition GenBank: BC009842
• Symbole du gène: NUDT6
• Identification du gène (NCBI): 11162
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NUDT6, also named as FGF2AS and GFG, belongs to the Nudix hydrolase family. It may contribute to the regulation of cell proliferation. NUDT6 maybe a candidate gene for fat deposition. The 72 kDa is a homodimer form of NUDT6.

Publications

Species	Application	Title
rat	WB,IF	J Physiol; FGF2 gene's antisense protein, NUDT6, plays a depressogenic role by promoting inflammation and suppressing neurogenesis without altering FGF2 signalling; Authors - Burak Uzay
human	WB	Mol Endocrinol; Regulation of fibroblast growth factor-2 by an endogenous antisense RNA and by argonaute-2.; Authors - MacFarlane Leigh-Ann LA
human	WB	Mol Carcinog; Regulation of FGF-2 by an endogenous antisense RNA: effects on cell adhesion and cell-cycle progression.; Authors - MacFarlane Leigh-Ann LA
mouse	WB	Cancer Sci; PMID: 29532562; Authors - Ji-Chang Wang
rat	WB	Genes (Basel); Regulation of fibroblast growth factor-2 expression and cell cycle progression by an endogenous antisense RNA.; Authors - Mark Baguma-Nibasheka ; KD Validated
human	WB	Wound Repair Regen; GHK-Cu-liposomes accelerate scald wound healing in mice by promoting cell proliferation and angiogenesis.; Authors - Xinying Wang

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