Anticorps Polyclonal de lapin anti-NUDT8

NUDT8 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 16098-1-AP

Synonymes

EC:3.6.1.-, Mitochondrial coenzyme A diphosphatase NUDT8, Nucleoside diphosphate-linked moiety X motif 8, Nudix motif 8

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Galerie de données de validation

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  WB analysis using 16098-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16098-1-AP (NUDT8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16098-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16098-1-AP (NUDT8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16098-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16098-1-AP (NUDT8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human colon cancer using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16098-1-AP (NUDT8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16098-1-AP (NUDT8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16098-1-AP (NUDT8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16098-1-AP (NUDT8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 16098-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Jurkat,
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu cardiaque de souris, tissu cardiaque humain, tissu cutané humain, tissu hépatique humain, tissu ovarien humain, tissu rénal humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

16098-1-AP cible NUDT8 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NUDT8 Protéine recombinante Ag8945
• Nom complet: nudix (nucleoside diphosphate linked moiety X)-type motif 8
• Masse moléculaire calculée: 236 aa, 25 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC018644
• Symbole du gène: NUDT8
• Identification du gène (NCBI): 254552
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NUDT8 belongs to the Nudix hydrolase family, which is involved in intracellular metabolic processes, especially related to the decomposition and degradation of nucleotides. NUDT8 is a novel CoA diphosphohydrolase that localizes to the mitochondria and is broadly expressed in mitochondria-rich organs such as the heart, kidneys, liver, and brown adipose tissue(PMID: 31004344).

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