Anticorps Polyclonal de lapin anti-NUFIP1

• Phare
• Validé par KD/KO

NUFIP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12515-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis of HeLa using 12515-1-AP (same clone as 12515-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12515-1-AP (NUFIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12515-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A375 using 12515-1-AP (same clone as 12515-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 12515-1-AP (NUFIP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12515-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 12515-1-AP (same clone as 12515-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12515-1-AP (NUFIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12515-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 12515-1-AP (same clone as 12515-1-PBS)

  IP result of anti-NUFIP1 (IP:12515-1-AP, 4ug; Detection:12515-1-AP 1:800) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 12515-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 12515-1-AP (same clone as 12515-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12515-1-AP (NUFIP1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12515-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 12515-1-AP (same clone as 12515-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12515-1-AP (NUFIP1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12515-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 12515-1-AP (same clone as 12515-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUFIP1 antibody (12515-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12515-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

12515-1-PBS cible NUFIP1 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NUFIP1 Protéine recombinante Ag3197
• Nom complet: nuclear fragile X mental retardation protein interacting protein 1
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC017745
• Symbole du gène: NUFIP1
• Identification du gène (NCBI): 26747
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Fragile X syndrome, the most common cause of inherited mental retardation, is caused by the absence of FMRP (Fragile X Mental Retardation Protein). FMRP is an RNA binding protein reported to be involved in translational control, notably at postsynaptic sites of protein synthesis as a part of a multiprotein/mRNA complex[PMID:12941608]. NUFIP1 is one of the several FMRP-interacting proteins. NUFIP can act as a pol II-specific basal transcriptional activator in vitro and when ectopically overexpressed in vivo. NUFIP can directly activate promoters by enhancing the ATP-dependent release of hyperphosphorylated form of pol II from open transcription complexes[PMID:15107825].

{{ptg:RelatedPrimaryAntibodies}}