Anticorps Polyclonal de lapin anti-NUFIP2

NUFIP2 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 17752-1-AP

Synonymes

182 FIP, 82 FIP, 82 kDa FMRP-interacting protein, 82-FIP, Cell proliferation-inducing gene 1 protein

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Galerie de données de validation

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  WB analysis of HeLa using 17752-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17752-1-AP (NUFIP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17752-1-AP

  IP result of anti-NUFIP2 (IP:17752-1-AP, 4ug; Detection:17752-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human cerebellum using 17752-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 17752-1-AP (NUFIP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cerebellum using 17752-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 17752-1-AP (NUFIP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa
• Résultats positifs en IP: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

17752-1-AP cible NUFIP2 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NUFIP2 Protéine recombinante Ag12094
• Nom complet: nuclear fragile X mental retardation protein interacting protein 2
• Masse moléculaire calculée: 695 aa, 76 kDa
• Poids moléculaire observé: 76-82 kDa
• Numéro d’acquisition GenBank: BC108307
• Symbole du gène: NUFIP2
• Identification du gène (NCBI): 57532
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IF	Cell; Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization.; Authors - David W Sanders
mouse	IHC	Proc Natl Acad Sci U S A; Succinyl-CoA ligase ADP-forming subunit beta promotes stress granule assembly to regulate redox and drive cancer metastasis; Authors - Austin C Boese
human	WB	J Cell Biol; Proteomic and 3D structure analyses highlight the C/D box snoRNP assembly mechanism and its control.; Authors - Jonathan Bizarro
		Neuron; Increased G3BP2-Tau interaction in tauopathies is a natural defense against Tau aggregation; Authors - Congwei Wang
		Cell Rep; The divergent effects of G3BP orthologs on human stress granule assembly imply a centric role for the core protein interaction network; Authors - Zhiying Yao
mouse	WB,IF	Neurobiol Dis; ATXN2L primarily interacts with NUFIP2, the absence of ATXN2L results in NUFIP2 depletion, and the ATXN2-polyQ expansion triggers NUFIP2 accumulation; Authors - Jana Key

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