Anticorps Polyclonal de lapin anti-NUMB

• Phare
• Validé par KD/KO

NUMB Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, FC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 18701-1-AP

Synonymes

h Numb, NUMB, numb homolog (Drosophila), Protein numb homolog, Protein S171, S171

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Galerie de données de validation

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  WB analysis using 18701-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 18701-1-AP (NUMB antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 18701-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18701-1-AP (NUMB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 18701-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18701-1-AP (NUMB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 18701-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18701-1-AP (NUMB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HepG2 using 18701-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug NUMB antibody (18701-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules A431, tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below

Informations sur le produit

18701-1-AP cible NUMB dans les applications de WB, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: numb homolog (Drosophila)
• Masse moléculaire calculée: 71 kDa
• Poids moléculaire observé: 66-72 kDa
• Numéro d’acquisition GenBank: BC068476
• Symbole du gène: NUMB
• Identification du gène (NCBI): 8650
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NUMB, also named as S171, plays a role in the determination of cell fates during development. It is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. The degradation of NUMB is induced in a proteasome-dependent manner by MDM2. Four transcript variants encoding different isoforms have been found for this gene. The antibody can bind to all these four isoforms P65, P66, P71 and P72, can't recognize the NUMBL.

Publications

Species	Application	Title
human	WB	Cancer Res; Regulation of lung cancer metastasis by Klf4-Numb-like signaling.; Authors - Vaira Valentina V ; KD Validated
human	WB	Cancer Lett; Therapeutic targeting m6A-guided miR-146a-5p signaling contributes to the melittin-induced selective suppression of bladder cancer.; Authors - Rucheng Yan
Human	IHC	Front Cell Dev Biol; IFT20 Mediates the Transport of Cell Migration Regulators From the Trans-Golgi Network to the Plasma Membrane in Breast Cancer Cells.; Authors - Huihui Yang
human	WB	Neurobiol Dis; The polarity protein Par3 regulates APP trafficking and processing through the endocytic adaptor protein Numb.; Authors - Miao Sun ; KD Validated
human	WB,IHC	J Cell Mol Med; Musashi2 predicts poor prognosis and invasion in hepatocellular carcinoma by driving epithelial-mesenchymal transition.; Authors - Lu He
human	WB	Oncol Lett; A gene mutation in RNA-binding protein 10 is associated with lung adenocarcinoma progression and poor prognosis.; Authors - Lin-Lin Yin