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Anticorps Monoclonal anti-NUMB

NUMB Monoclonal Antibody for ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

ELISA

Conjugaison

Non conjugué

CloneNo.

1A8A1

N° de cat : 60137-2-Ig

Synonymes

Galerie de données de validation

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  • Western Blot (WB) analysis of A431 cells using NUMB Monoclonal antibody (60137-2-Ig)

    WB analysis of A431 using 60137-2-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 60137-2-Ig (NUMB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using NUMB Monoclonal antibody (60137-2-Ig)

    WB analysis of A431 using 60137-2-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 60137-2-Ig (NUMB antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using NUMB Monoclonal antibody (60137-2-Ig)

    WB analysis of A431 using 60137-2-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 60137-2-Ig (NUMB antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

Applications testées

Dilution recommandée

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

60137-2-Ig cible NUMB dans les applications de ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène Peptide
Nom complet numb homolog (Drosophila)
Masse moléculaire calculée 71 kDa
Numéro d’acquisition GenBankBC068476
Symbole du gène NUMB
Identification du gène (NCBI) 8650
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NUMB, also named as S171, plays a role in the determination of cell fates during development. It is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. The degradation of NUMB is induced in a proteasome-dependent manner by MDM2. Four transcript variants encoding different isoforms have been found for this gene. The antibody can bind to all these four isoforms P65, P66, P71 and P72, can’t recognize the NUMBL.