Anticorps Monoclonal anti-NUMBL

NUMBL Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain et plus (1)

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications(15)

CloneNo.

4C10C5

Avis (1)

N° de cat : 60009-1-Ig

Synonymes

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Galerie de données de validation

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  WB analysis of HepG2 using 60009-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60009-1-Ig (NUMBL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IP: See 1 publications below

Informations sur le produit

60009-1-Ig cible NUMBL dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NUMBL Protéine recombinante Ag0156
• Nom complet: numb homolog (Drosophila)-like
• Masse moléculaire calculée: 65 kDa
• Numéro d’acquisition GenBank: BC001794
• Symbole du gène: NUMBL
• Identification du gène (NCBI): 9253
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Précipitation de l'acide caprylique-du sulfate d'ammonium
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Numb-like (Numbl or Nbl) binds Notch and is an intracellular modulator of Notch signaling pathway. Numbl contains a phosphotyrosine binding (PTB) domain that could bind the Y682 motif present in the cytoplasmic tail of APP. Deduced molecular weight of Numbl is 72 kDa.

Publications

Species	Application	Title
		Elife; Host-derived Lactobacillus plantarum alleviates hyperuricemia by improving gut microbial community and hydrolase-mediated degradation of purine nucleosides; Authors - Yang Fu
		Elife; Alpha protocadherins and Pyk2 kinase regulate cortical neuron migration and cytoskeletal dynamics via Rac1 GTPase and WAVE complex in mice.; Authors - Li Fan
Mouse	WB	Commun Biol; STAT1 mediates the pro-inflammatory role of GBP5 in colitis; Authors - Yichen Li
	WB	Cells; PBRM1 Cooperates with YTHDF2 to Control HIF-1α Protein Translation.; Authors - Alena Shmakova
	WB	Oncol Lett; DNMT3B regulates proliferation of A549 cells through the microRNA-152-3p/NCAM1 pathway.; Authors - Bo Yang
human	WB	J Oncol; Plumbagin Enhances the Radiosensitivity of Tongue Squamous Cell Carcinoma Cells via Downregulating ATM.; Authors - Shu-Ting Pan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aleksandr (Verified Customer) (01-13-2023)

Second band was found in some samples.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Drosophila ovaries

NUMBL Antibody Western Blot validation (1:5000 dilution) in Drosophila ovaries (Cat no:60009-1-Ig)

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