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Anticorps Monoclonal anti-NUMBL

NUMBL Monoclonal Antibody for WB,ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

4C10C5

N° de cat : 60009-1-Ig

Synonymes

Galerie de données de validation

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  • Western Blot (WB) analysis of HepG2 cells using NUMBL Monoclonal antibody (60009-1-Ig)

    WB analysis of HepG2 using 60009-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 60009-1-Ig (NUMBL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Applications testées

Résultats positifs en WBcellules HepG2

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

WBSee 2 publications below

Informations sur le produit

60009-1-Ig cible NUMBL dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Réactivité citéeHumain
Hôte / Isotype Mouse / IgG2a
Clonalité Monoclonal
Type Anticorps
Immunogène NUMBL Protéine recombinante Ag0156
Nom complet numb homolog (Drosophila)-like
Masse moléculaire calculée 65 kDa
Numéro d’acquisition GenBankBC001794
Symbole du gène NUMBL
Identification du gène (NCBI) 9253
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Précipitation de l'acide caprylique/du sulfate d'ammonium
Tampon de stockage PBS avec azoture de sodium à 0,1 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Numb-like (Numbl or Nbl) binds Notch and is an intracellular modulator of Notch signaling pathway. Numbl contains a phosphotyrosine binding (PTB) domain that could bind the Y682 motif present in the cytoplasmic tail of APP. Deduced molecular weight of Numbl is 72 kDa.

Publications

SpeciesApplicationTitle
humanWB

J Oncol

Plumbagin Enhances the Radiosensitivity of Tongue Squamous Cell Carcinoma Cells via Downregulating ATM.

Authors - Shu-Ting Pan
View Article
humanWB

J Transl Med

Irradiation-induced exosomal HMGB1 to confer radioresistance via the PI3K/AKT/FOXO3A signaling pathway in ESCC

Authors - Xingyu Du
View Article

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Aleksandr (Verified Customer) (01-13-2023)

Second band was found in some samples.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Drosophila ovaries
NUMBL Antibody Western Blot validation (1:5000 dilution) in Drosophila ovaries (Cat no:60009-1-Ig)