Anticorps Polyclonal de lapin anti-NUP107

NUP107 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(12)

Avis (1)

N° de cat : 19217-1-AP

Synonymes

107 kDa nucleoporin, nucleoporin 107kDa, Nucleoporin Nup107, NUP107, NUP84

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Galerie de données de validation

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  WB analysis using 19217-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19217-1-AP (NUP107 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 19217-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 19217-1-AP (NUP107 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 19217-1-AP

  IP result of anti-NUP107 (IP:19217-1-AP, 4ug; Detection:19217-1-AP 1:2000) with HeLa cells lysate 1320 ug.

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HeLa, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IP: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

19217-1-AP cible NUP107 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NUP107 Protéine recombinante Ag5250
• Nom complet: nucleoporin 107kDa
• Masse moléculaire calculée: 106 kDa
• Poids moléculaire observé: 107 kDa
• Numéro d’acquisition GenBank: BC017167
• Symbole du gène: NUP107
• Identification du gène (NCBI): 57122
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IF	Nat Commun; The master energy homeostasis regulator PGC-1α exhibits an mRNA nuclear export function; Authors - Simeon R Mihaylov
rat	WB	Neuron; Nucleoporin Seh1 Interacts with Olig2/Brd7 to Promote Oligodendrocyte Differentiation and Myelination.; Authors - Zhixiong Liu
mouse	WB	J Clin Invest; Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.; Authors - Zhixiong Liu
		Dev Cell; Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination; Authors - Li Li
		Dev Cell; Nucleoporin Seh1 controls murine neocortical development via transcriptional repression of p21 in neural stem cells; Authors - Wenxiu Dai
mouse	WB	Elife; Suppression of C9orf72 RNA repeat-induced neurotoxicity by the ALS-associated RNA-binding protein Zfp106.; Authors - Barbara Celona

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Barbara (Verified Customer) (03-28-2022)

Reliable antibody for western blot detection of NUP-107

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Neuro-2A lysates

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