Anticorps Polyclonal de lapin anti-NUP153

NUP153 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 14189-1-AP

Synonymes

153 kDa nucleoporin, HNUP153, N153, nucleoporin 153kDa, Nucleoporin Nup153, NUP153

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Galerie de données de validation

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  WB analysis of K-562 using 14189-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14189-1-AP (NUP153 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 14189-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14189-1-AP (NUP153 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 14189-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 14189-1-AP (NUP153 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 14189-1-AP

  IP result of anti-NUP153 (IP:14189-1-AP, 4ug; Detection:14189-1-AP 1:500) with K-562 cells lysate 3600ug.

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules A431, cellules HeLa, cellules HepG2, cellules MCF-7
• Résultats positifs en IP: cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

14189-1-AP cible NUP153 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NUP153 Protéine recombinante Ag5519
• Nom complet: nucleoporin 153kDa
• Masse moléculaire calculée: 154 kDa
• Poids moléculaire observé: 154 kDa
• Numéro d’acquisition GenBank: BC052965
• Symbole du gène: NUP153
• Identification du gène (NCBI): 9972
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NUP153 is an FG-repeat containing NUP, contains a high-affinity site for importin-β, localizes on the nucleoplasmic face of the nuclear pore and is required for assembly of the nuclear basket and import of a subset of nuclear proteins [PMID:18845677]. NUP153 also facilitates nuclear export of mRNA and ribonucleoprotein particles and may have additional functions within the nucleus [PMID:16195343]. The 53BP1-NUP153/importin-β pathway as an important aspect of the DDR network add to an emerging evidence of subcellular trafficking as an integral part of genome surveillance [PMID:22075984].

Publications

Species	Application	Title
human	WB	Biotechnol J; Highly efficient identification of nucleocytoplasmic O-glycosylation by the TurboID-based proximity labeling method in living cells; Authors - Yunfeng Long
pig	WB	Int J Biol Macromol; Subcellular proteomics reveals the crosstalk between nucleocytoplasmic trafficking and the innate immune response to Senecavirus A infection; Authors - Mingyang Li
Mouse		Nat Commun; BOOST: a robust ten-fold expansion method on hour-scale; Authors - Jinyu Guo
human	WB	J Med Virol; Novel Function of NUP153 in HNF4α Transcriptional Upregulation Contributes to Promoting HBV Replication; Authors - Qianqian Jiang

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