Anticorps Polyclonal de lapin anti-NUP153

NUP153 Polyclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14189-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of K-562 using 14189-1-AP (same clone as 14189-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 14189-1-AP (NUP153 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14189-1-PBS in a different storage buffer formulation.

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  WB analysis using 14189-1-AP (same clone as 14189-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14189-1-AP (NUP153 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14189-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 14189-1-AP (same clone as 14189-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 14189-1-AP (NUP153 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14189-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 14189-1-AP (same clone as 14189-1-PBS)

  IP result of anti-NUP153 (IP:14189-1-AP, 4ug; Detection:14189-1-AP 1:500) with K-562 cells lysate 3600ug. This data was developed using the same antibody clone with 14189-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14189-1-PBS cible NUP153 dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NUP153 Protéine recombinante Ag5519
• Nom complet: nucleoporin 153kDa
• Masse moléculaire calculée: 154 kDa
• Poids moléculaire observé: 154 kDa
• Numéro d’acquisition GenBank: BC052965
• Symbole du gène: NUP153
• Identification du gène (NCBI): 9972
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NUP153 is an FG-repeat containing NUP, contains a high-affinity site for importin-β, localizes on the nucleoplasmic face of the nuclear pore and is required for assembly of the nuclear basket and import of a subset of nuclear proteins [PMID:18845677]. NUP153 also facilitates nuclear export of mRNA and ribonucleoprotein particles and may have additional functions within the nucleus [PMID:16195343]. The 53BP1-NUP153/importin-β pathway as an important aspect of the DDR network add to an emerging evidence of subcellular trafficking as an integral part of genome surveillance [PMID:22075984].

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