Anticorps Recombinant de lapin anti-NUP153

NUP153 Recombinant Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251519G11

N° de cat : 86622-1-PBS

Synonymes

153 kDa nucleoporin, HNUP153, N153, nucleoporin 153kDa, Nucleoporin Nup153

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Galerie de données de validation

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  WB analysis using 86622-1-RR (same clone as 86622-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86622-1-RR (NUP153 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86622-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP02679-1

  The mean NUP153 concentration was determined to be 30.07 ng/mL in K-562 cell extract based on a 1.20 mg/mL extract load, 97.51 ng/mL in HepG2 cell extract based on a 1.20 mg/mL extract load and 33.07 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02679-1

  Sandwich ELISA standard curve of MP02679-1, Human NUP153 Recombinant Matched Antibody Pair - PBS only. 86622-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag5519. 86622-2-PBS was HRP conjugated as the detection antibody. Range: 1.56-100 ng/mL.

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  IF Staining of HeLa using 86622-1-RR (same clone as 86622-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUP153 antibody (86622-1-RR, Clone: 251519G11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86622-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86622-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86622-1-RR against Human NUP153 were performed. The affinity constant is 2.32 nM.

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Informations sur le produit

86622-1-PBS cible NUP153 dans les applications de WB, IF/ICC, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NUP153 Protéine recombinante Ag5519
• Nom complet: nucleoporin 153kDa
• Masse moléculaire calculée: 154 kDa
• Poids moléculaire observé: 160-170 kDa
• Numéro d’acquisition GenBank: BC052965
• Symbole du gène: NUP153
• Identification du gène (NCBI): 9972
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NUP153 is an FG-repeat containing NUP, contains a high-affinity site for importin-β, localizes on the nucleoplasmic face of the nuclear pore and is required for assembly of the nuclear basket and import of a subset of nuclear proteins [PMID:18845677]. NUP153 also facilitates nuclear export of mRNA and ribonucleoprotein particles and may have additional functions within the nucleus [PMID:16195343]. The 53BP1-NUP153/importin-β pathway as an important aspect of the DDR network add to an emerging evidence of subcellular trafficking as an integral part of genome surveillance [PMID:22075984].

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