Anticorps Polyclonal de lapin anti-NUP205

NUP205 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 24439-1-AP

Synonymes

205 kDa nucleoporin, C7orf14, KIAA0225, nucleoporin 205kDa, Nucleoporin Nup205, NUP205

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Galerie de données de validation

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  WB analysis of HeLa using 24439-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24439-1-AP (NUP205 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 24439-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24439-1-AP (NUP205 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 24439-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24439-1-AP (NUP205 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 24439-1-AP

  IP result of anti-NUP205 (IP:24439-1-AP, 4ug; Detection:24439-1-AP 1:1500) with HeLa cells lysate 4000ug.

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  IHC staining of human stomach using 24439-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach slide using 24439-1-AP (NUP205 Antibody) at dilution of 1:50.

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  IHC staining of human stomach using 24439-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach slide using 24439-1-AP (NUP205 Antibody) at dilution of 1:50.

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules Jurkat
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu d'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

24439-1-AP cible NUP205 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NUP205 Protéine recombinante Ag19832
• Nom complet: nucleoporin 205kDa
• Masse moléculaire calculée: 2012 aa, 228 kDa
• Poids moléculaire observé: 205 kDa
• Numéro d’acquisition GenBank: BC136624
• Symbole du gène: NUP205
• Identification du gène (NCBI): 23165
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NUP205, also named as C7orf14 and KIAA0225, is a 205 kDa nucleoporin. It plays a role in the nuclear pore complex (NPC) assembly and/or maintenance.

Publications

Species	Application	Title
human	IF	Nat Methods; Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM).; Authors - Marine H Laporte
mouse	WB	J Clin Invest; Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.; Authors - Zhixiong Liu
		Dev Cell; Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination; Authors - Li Li
human	WB	Oncogene; DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity.; Authors - Bin Chen
human	WB	Hum Mutat; Lamin A mutation impairs interaction with nucleoporin NUP155 and disrupts nucleocytoplasmic transport in atrial fibrillation.; Authors - Meng Han
human	WB,CoIP	Int Immunopharmacol; EGFLAM exhibits oncogenic activity and shows promise as a prognostic biomarker and therapeutic target in glioblastoma; Authors - Kaishu Li

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