Anticorps Recombinant de lapin anti-NUP214

NUP214 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242782H2

N° de cat : 85161-4-PBS

Synonymes

242782H2, CAIN, CAN, KIAA0023

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Galerie de données de validation

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  WB analysis using 85161-4-RR (same clone as 85161-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85161-4-RR (NUP214 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85161-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 85161-4-RR (same clone as 85161-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85161-4-RR (NUP214 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85161-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 85161-4-RR (same clone as 85161-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85161-4-RR (NUP214 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85161-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 85161-4-RR (same clone as 85161-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85161-4-RR (NUP214 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85161-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 85161-4-RR (same clone as 85161-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85161-4-RR (NUP214 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85161-4-PBS in a different storage buffer formulation.

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  IF Staining of K-562 using 85161-4-RR (same clone as 85161-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using NUP214 antibody (85161-4-RR, Clone: 242782H2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85161-4-PBS in a different storage buffer formulation.

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Informations sur le produit

85161-4-PBS cible NUP214 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NUP214 Protéine recombinante Ag21377
• Nom complet: nucleoporin 214kDa
• Masse moléculaire calculée: 2080 aa, 213 kDa
• Poids moléculaire observé: 250 kDa
• Numéro d’acquisition GenBank: BC105998
• Symbole du gène: NUP214
• Identification du gène (NCBI): 8021
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NUP214 is also known as CAN and is an FG nucleoporin anchored to the cytoplasmic ring of the nuclear pore complex (NPC) and forms a subcomplex with nucleoporin NUP88. NUP214 is a component of the NPC with a key role in protein and mRNA nuclear export. NUP214 is a target for chromosomal translocations involved in leukemogenesis (PMID: 17264208, 30669574). NUP214 is expressed in thymus, spleen, bone marrow, kidney, brain, and testis, but hardly in all other tissues or in whole embryos during development. The observed molecular weight of NUP214 is 250 kDa, which is consistent with the description in the literature (PMID: 12191473).

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