Anticorps Monoclonal anti-NUP50

• Phare
• Validé par KD/KO

NUP50 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

1D5A7

N° de cat : 67001-1-Ig

Synonymes

1D5A7, 50 kDa nucleoporin, Nuclear pore-associated protein 60 kDa-like, nucleoporin 50kDa, Nucleoporin Nup50

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Galerie de données de validation

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  WB analysis using 67001-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67001-1-Ig (NUP50 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of HEK-293 using 67001-1-Ig

  WB result of NUP50 antibody (67001-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUP50 transfected HEK-293 cells.

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  WB analysis using 67001-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67001-1-Ig (NUP50 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67001-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67001-1-Ig (NUP50 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67001-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67001-1-Ig (NUP50 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67001-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67001-1-Ig (NUP50 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HCT 116, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HT-29, cellules Jurkat, cellules K-562, cellules MCF-7, cellules MOLT-4, cellules Ramos, cellules Sp2/0, cellules U2OS, cels Daudi
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67001-1-Ig cible NUP50 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NUP50 Protéine recombinante Ag15144
• Nom complet: nucleoporin 50kDa
• Masse moléculaire calculée: 468 aa, 50 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC028125
• Symbole du gène: NUP50
• Identification du gène (NCBI): 10762
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nup50 (also known as Npap60) is a nucleoporin that binds directly to importin α and their interaction plays an important role in nuclear import. Nup50 is a mobile nucleoporin localized primarily in the nuclear basket of the nuclear pore complex (NPC) and in the nucleoplasm. Human Nup50 has two isoforms: Npap60L (1-469 aa), and its alternatively spliced isoform, Npap60S (29-469 aa) of Npap60L.

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