Anticorps Polyclonal de lapin anti-NUP54

NUP54 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 16232-1-AP

Synonymes

Nucleoporin p54, nucleoporin 54kDa, 54 kDa nucleoporin

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Galerie de données de validation

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  WB analysis of HeLa using 16232-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16232-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16232-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16232-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 16232-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 16232-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 16232-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16232-1-AP (NUP54 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16232-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16232-1-AP (NUP54 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules HepG2, cellules K-562, tissu cardiaque de souris, tissu hépatique de souris
• Résultats positifs en IHC: tissu d'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:300-1:2000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below

Informations sur le produit

16232-1-AP cible NUP54 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NUP54 Protéine recombinante Ag8882
• Nom complet: nucleoporin 54kDa
• Masse moléculaire calculée: 507 aa, 55 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC012559
• Symbole du gène: NUP54
• Identification du gène (NCBI): 53371
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
		Dev Cell; Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination; Authors - Li Li
human	WB	Elife; Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2.; Authors - Melissa Kane
human	WB	J Exp Clin Cancer Res; Transketolase (TKT) activity and nuclear localization promote hepatocellular carcinoma in a metabolic and a non-metabolic manner.; Authors - Zhaoyu Qin
mouse	WB	Cell Rep; Nucleoporin Seh1 maintains Schwann cell homeostasis by regulating genome stability and necroptosis; Authors - Mei Wu
human	WB	bioRxiv; Global mapping of the Chlamydia trachomatis conventional secreted effector - host interactome reveals CebN interacts with nucleoporins and Rae1 to impede STAT1 nuclear translocation; Authors - Brianna Steiert
human	WB	Nat Phys; Structural anisotropy results in mechano-directional transport of proteins across nuclear pores; Authors - Fani Panagaki ; KD Validated

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