Anticorps Polyclonal de lapin anti-NUP54

NUP54 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16232-1-PBS

Synonymes

Nucleoporin p54, nucleoporin 54kDa, 54 kDa nucleoporin

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Galerie de données de validation

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  WB analysis of HeLa using 16232-1-AP (same clone as 16232-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16232-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 16232-1-AP (same clone as 16232-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16232-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 16232-1-AP (same clone as 16232-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16232-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 16232-1-AP (same clone as 16232-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16232-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 16232-1-AP (same clone as 16232-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16232-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart using 16232-1-AP (same clone as 16232-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16232-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 16232-1-AP (same clone as 16232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16232-1-AP (NUP54 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16232-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 16232-1-AP (same clone as 16232-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16232-1-AP (NUP54 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16232-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16232-1-PBS cible NUP54 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NUP54 Protéine recombinante Ag8882
• Nom complet: nucleoporin 54kDa
• Masse moléculaire calculée: 507 aa, 55 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC012559
• Symbole du gène: NUP54
• Identification du gène (NCBI): 53371
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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