Anticorps Polyclonal de lapin anti-NUP62

• Phare
• Validé par KD/KO

NUP62 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(19)

N° de cat : 13916-1-AP

Synonymes

SNDI, Nucleoporin Nup62, nucleoporin 62kDa, Nuclear pore glycoprotein p62, IBSN

---

Galerie de données de validation

• 

  WB analysis of PC-3 using 13916-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13916-1-AP (NUP62 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 13916-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13916-1-AP (NUP62 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of NIH/3T3 using 13916-1-AP

  IP result of anti-NUP62 (IP:13916-1-AP, 4ug; Detection:13916-1-AP 1:1000) with NIH/3T3 cells lysate 1200ug.

• 

  IHC staining of human stomach using 13916-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13916-1-AP (NUP62 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules PC-3, cellules NIH/3T3
• Résultats positifs en IP: cellules NIH/3T3
• Résultats positifs en IHC: tissu d'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 16 publications below
• IF: See 7 publications below
• IP: See 1 publications below

Informations sur le produit

13916-1-AP cible NUP62 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, poulet, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NUP62 Protéine recombinante Ag4912
• Nom complet: nucleoporin 62kDa
• Masse moléculaire calculée: 522 aa, 53 kDa
• Poids moléculaire observé: 62 kDa
• Numéro d’acquisition GenBank: BC014842
• Symbole du gène: NUP62
• Identification du gène (NCBI): 23636
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NUP62 belongs to the nucleoporin NSP1/NUP62 family. It is essential component of the nuclear pore complex. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is probably involved in protein-protein interaction via coiled-coil formation and may function in anchorage of p62 to the pore complex. Defects in NUP62 are the cause of infantile striatonigral degeneration (SNDI). NUP62 consists of a single promoter with a CpG island and three transcribed exons. It is ubiquitously expressed, an observation compatible with its essential role in transporting cargo across the nuclear envelope. (PMID:16029492)

Publications

Species	Application	Title
human	WB,IF	Nat Microbiol; Nuclear pore blockade reveals that HIV-1 completes reverse transcription and uncoating in the nucleus.; Authors - Adarsh Dharan
mouse	WB	Mol Cell; RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages; Authors - Teodorus Theo Susanto
mouse	WB	J Clin Invest; Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.; Authors - Zhixiong Liu
human	WB	PLoS Genet; Nucleoporin TPR promotes tRNA nuclear export and protein synthesis in lung cancer cells.; Authors - Miao Chen
mouse	WB	iScience; Tat-P combined with GAPR1 releases Beclin1 to promote autophagy and improve Bronchopulmonary dysplasia model; Authors - Yahui Zhou
rat	WB	Pain; Transcriptional repression of the M channel subunit Kv7.2 in chronic nerve injury.; Authors - Rose Kirstin K

{{ptg:RelatedPrimaryAntibodies}}