Anticorps Monoclonal anti-NUP62

NUP62 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2G12B3

N° de cat : 66573-1-PBS

Synonymes

Nucleoporin Nup62, nucleoporin 62kDa, Nuclear pore glycoprotein p62, IBSN, 62 kDa nucleoporin

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Galerie de données de validation

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  WB analysis using 66573-1-Ig (same clone as 66573-1-PBS)

  Various cells were subjected to SDS PAGE followed by western blot with 66573-1-Ig (NUP62 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66573-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66573-1-Ig (same clone as 66573-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66573-1-Ig (NUP62 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66573-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66573-1-Ig (same clone as 66573-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66573-1-Ig (NUP62 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66573-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66573-1-Ig (same clone as 66573-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66573-1-Ig (NUP62 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66573-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66573-1-PBS cible NUP62 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NUP62 Protéine recombinante Ag4912
• Nom complet: nucleoporin 62kDa
• Masse moléculaire calculée: 522 aa, 53 kDa
• Poids moléculaire observé: 62 kDa
• Numéro d’acquisition GenBank: BC014842
• Symbole du gène: NUP62
• Identification du gène (NCBI): 23636
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NUP62 belongs to the nucleoporin NSP1/NUP62 family. It is essential component of the nuclear pore complex. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is probably involved in protein-protein interaction via coiled-coil formation and may function in anchorage of p62 to the pore complex. Defects in NUP62 are the cause of infantile striatonigral degeneration (SNDI). NUP62 consists of a single promoter with a CpG island and three transcribed exons. It is ubiquitously expressed, an observation compatible with its essential role in transporting cargo across the nuclear envelope. (PMID:16029492)

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