Anticorps Polyclonal de lapin anti-NUP85

NUP85 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15027-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 15027-1-AP (same clone as 15027-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15027-1-AP (NUP85 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15027-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 15027-1-AP (same clone as 15027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15027-1-AP (NUP85 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15027-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 15027-1-AP (same clone as 15027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15027-1-AP (NUP85 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15027-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 15027-1-AP (same clone as 15027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15027-1-AP (NUP85 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15027-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 15027-1-AP (same clone as 15027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15027-1-AP (NUP85 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15027-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 15027-1-AP (same clone as 15027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15027-1-AP (NUP85 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15027-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 15027-1-AP (same clone as 15027-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NUP85 antibody (15027-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 15027-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 15027-1-AP (same clone as 15027-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NUP85 antibody (15027-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 15027-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15027-1-PBS cible NUP85 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NUP85 Protéine recombinante Ag7158
• Nom complet: nucleoporin 85kDa
• Masse moléculaire calculée: 75 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC000697
• Symbole du gène: NUP85
• Identification du gène (NCBI): 79902
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

This gene encodes a protein component of the Nup107-160 subunit of the nuclear pore complex. NPCs are key for nucleocytoplasmic transport, but also regulate the mitotic machinery, transcription and chromatin organization through transport-independent mechanisms. The NUP107-160 complex is acknowledged to contribute to the assembly and maintenance of the NPC structure. Members of this largest subcomplex of the NPC associate with kinetochores, mitotic spindles, centrosomes and mitotic checkpoint regulators for proper completion of the cell cycle . Downregulation of NUP107-160 subcomplex members resulted in defective cytokinesis, compromised microtubule structures, altered cytoskeletal dynamics, impaired chromosome segregation and differentiation. (PMID:34170319)

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