Anticorps Polyclonal de lapin anti-NUPR1

• Phare
• Validé par KD/KO

NUPR1 Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications(20)

N° de cat : 15056-1-AP

Synonymes

NUPR 1, Nuclear protein 1, COM1

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Galerie de données de validation

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  IHC staining of human colon cancer using 15056-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15056-1-AP (NUPR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15056-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15056-1-AP (NUPR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15056-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15056-1-AP (NUPR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15056-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15056-1-AP (NUPR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15056-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15056-1-AP (NUPR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15056-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15056-1-AP (NUPR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer de la prostate humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 16 publications below
• IHC: See 9 publications below
• IF: See 4 publications below
• CoIP: See 2 publications below

Informations sur le produit

15056-1-AP cible NUPR1 dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NUPR1 Protéine recombinante Ag7039
• Nom complet: nuclear protein 1
• Masse moléculaire calculée: 8 kDa
• Numéro d’acquisition GenBank: BC002434
• Symbole du gène: NUPR1
• Identification du gène (NCBI): 26471
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NUPR1, also named as COM1 and P8, is a small nuclear protein that is responsive to various stress stimuli. NUPR1 can be induced by hypoxia, oxidative stress, while the expression is low in physiological condition. It is associated with chemoresistance in multiple malignancies. The predicted MW of NUPR1 is 8 kDa and we also detectet bands around 17 and 38 kDa, which are similar to the papers(PMID: 29130426, 27336713, 28694771, 22961798). NUPR1 was also found in a complex with ANXA2, TRIM21, YBX1, S100A9, HSPA8, HSPA9, HSPA4, HSPA5, and ESR1, suggesting that these proteins might play a role in its functional regulation (PMID: 33542201), and it can also form a complex with PADI4 and plays crucial functions in modulating DNA-repair, favoring metastasis, or facilitating citrullination of other proteins (PMID: 36858171).

Publications

Species	Application	Title
human,mouse	WB,IHC,IF,CoIP	BMC Med; NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma; Authors - Yizhi Zhan ; KD Validated
human	WB,IF	J Cell Physiol; Mechanistic study of NUPR1 in bladder cancer development through transcriptional regulation of CCR2; Authors - Zebin Shi
human	WB	J Inorg Biochem; Chronic nickel exposure alters extracellular vesicles to mediate cancer progression via sustained NUPR1 expression; Authors - Shan Liu ; KD Validated
human	WB,IHC	Discov Oncol; ATF4/NUPR1 axis promotes cancer cell survival and mediates immunosuppression in clear cell renal cell carcinoma; Authors - Yongliang Lu
human,mouse	WB,IHC	Pathol Res Pract; NUPR1 induces autophagy and promotes the progression of Esophageal squamous cell carcinoma via the MAPK-mTOR pathway; Authors - Shiheng Ren
human	WB	PLoS One; Adenomatous Polyposis Coli loss controls cell cycle regulators and response to paclitaxel in MDA-MB-157 metaplastic breast cancer cells.; Authors - Emily M Astarita

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