Anticorps Recombinant de lapin anti-NUPR1

NUPR1 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240149G4

N° de cat : 83315-2-RR

Synonymes

NUPR 1, Nuclear protein 1, COM1, 240149G4

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Galerie de données de validation

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  WB analysis of NCI-H1299 using 83315-2-RR

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 83315-2-RR (NUPR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83315-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83315-2-RR against Human NUPR1 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules NCI-H1299,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83315-2-RR cible NUPR1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NUPR1 Protéine recombinante Ag7039
• Nom complet: nuclear protein 1
• Masse moléculaire calculée: 8 kDa
• Poids moléculaire observé: 10 kDa
• Numéro d’acquisition GenBank: BC002434
• Symbole du gène: NUPR1
• Identification du gène (NCBI): 26471
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NUPR1, also named as COM1 and P8, is a small nuclear protein that is responsive to various stress stimuli. NUPR1 can be induced by hypoxia, oxidative stress, while the expression is low in physiological condition. It is associated with chemoresistance in multiple malignancies. The predicted MW of NUPR1 is 8 kDa, it also has another isoform of 10 kDa and we also detectet bands around 17 and 38 kDa, which are similar to the papers(PMID: 29130426, 27336713, 28694771, 22961798). NUPR1 was also found in a complex with ANXA2, TRIM21, YBX1, S100A9, HSPA8, HSPA9, HSPA4, HSPA5, and ESR1, suggesting that these proteins might play a role in its functional regulation (PMID: 33542201), and it can also form a complex with PADI4 and plays crucial functions in modulating DNA-repair, favoring metastasis, or facilitating citrullination of other proteins (PMID: 36858171).

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