Anticorps Polyclonal de lapin anti-NUSAP1

• Phare
• Validé par KD/KO

NUSAP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12024-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 12024-1-AP (same clone as 12024-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12024-1-AP (NUSAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12024-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 12024-1-AP (same clone as 12024-1-PBS)

  IP result of anti-NUSAP1 (IP:12024-1-AP, 4ug; Detection:12024-1-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 12024-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 12024-1-AP (same clone as 12024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12024-1-AP (NUSAP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12024-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 12024-1-AP (same clone as 12024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12024-1-AP (NUSAP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12024-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 12024-1-AP (same clone as 12024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12024-1-AP (NUSAP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12024-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 12024-1-AP (same clone as 12024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12024-1-AP (NUSAP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12024-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 12024-1-AP (same clone as 12024-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUSAP1 antibody (12024-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12024-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12024-1-PBS cible NUSAP1 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NUSAP1 Protéine recombinante Ag2654
• Nom complet: nucleolar and spindle associated protein 1
• Masse moléculaire calculée: 440 aa, 49 kDa
• Poids moléculaire observé: 47-52 kDa
• Numéro d’acquisition GenBank: BC001308
• Symbole du gène: NUSAP1
• Identification du gène (NCBI): 51203
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NUSAP1, also named as ANKT, belongs to the NUSAP family. It is microtubule-associated protein with the capacity to bundle and stabilize microtubules. NUSAP1 may associate with chromosomes and promote the organization of mitotic spindle microtubules around them. NUSAP1 plays a role in connecting apoptosis with cell migration and it is a potentially involved in morphogenesis in vertebrates (PMID:21203972). NUSAP1 is an immunogenic antigen in 65% of patients with AML following allogeneic HCT and suggests a tumor antigen role(PMID:20053754 ). NuSAP1 protein level is tightly regulated by the APC/C ubiquitin ligase complex and NuSAP induces mitotic arrest dependent of its microtubule affinity (PMID:17618083). NUSAP1 is overexpressed in pituitary adenomas.

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