Anticorps Monoclonal anti-NVL

NVL Monoclonal Antibody for WB, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D8G4

N° de cat : 68399-1-PBS

Synonymes

1D8G4, Nuclear valosin-containing protein-like, nuclear VCP like, Nuclear VCP like protein, Nuclear VCP-like protein

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Galerie de données de validation

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  WB analysis using 68399-1-Ig (same clone as 68399-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68399-1-Ig (NVL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68399-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 68399-1-Ig (same clone as 68399-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.2 ug NVL Monoclonal antibody (68399-1-Ig, Clone:1D8G4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.2 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68399-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 68399-1-PBS

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug NVL Monoclonal antibody, PBS Only (68399-1-PBS, Clone:1D8G4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68399-1-PBS cible NVL dans les applications de WB, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NVL Protéine recombinante Ag9321
• Nom complet: nuclear VCP-like
• Masse moléculaire calculée: 856aa,95 kDa; 659aa,73 kDa
• Poids moléculaire observé: 95 kDa
• Numéro d’acquisition GenBank: BC012105
• Symbole du gène: NVL
• Identification du gène (NCBI): 4931
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NVL(nuclear VCP-like) belongs to the AAA ATPase family and may facilitate the dissociation and recycling of nucleolin, thereby promoting efficient ribosome biogenesis. It acts as an ATP-dependent zinc metallopeptidase. This protein has 5 isoforms produced by alternative splicing.

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