Anticorps Polyclonal de lapin anti-NXN

• Phare
• Validé par KD/KO

NXN Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 16128-1-AP

Synonymes

NRX, nucleoredoxin, NXN, TRG 4

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Galerie de données de validation

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  WB analysis of HeLa using 16128-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16128-1-AP (NXN antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis using 16128-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16128-1-AP (NXN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 16128-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16128-1-AP (NXN antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16128-1-AP

  IP Result of anti-NXN (IP:16128-1-AP, 4ug; Detection:16128-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of human colon cancer using 16128-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16128-1-AP (NXN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16128-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16128-1-AP (NXN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 16128-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NXN antibody (16128-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules DU 145, cellules HCT 116, cellules HEK-293, cellules HeLa, cellules HepG2, cellules SH-SY5Y, tissu cardiaque humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

16128-1-AP cible NXN dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NXN Protéine recombinante Ag9113
• Nom complet: nucleoredoxin
• Masse moléculaire calculée: 435 aa, 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC009327
• Symbole du gène: NXN
• Identification du gène (NCBI): 64359
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nucleoredoxin (NRX/NXN), a relatively uncharacterized member of the TRX family protein, has recently been reported to regulate the Wnt/β-catenin pathway, which itself regulates cell fate and early development, in a redox-dependent manner(PMID:17567240). Northern blot analysis detected several nucleoredoxin splice variants in adult mouse tissues, with highest expression in testis and skin and lowest expression in liver(PMID:9119370). It has 3 isoforms produced by alternative splicing. This antibody is specific to NXN.

Publications

Species	Application	Title
mouse	WB	Sci Signal; Mitochondrial reactive oxygen species promote epidermal differentiation and hair follicle development.; Authors - Hamanaka Robert B RB
human	WB	Free Radic Biol Med; Oxidative stress as a candidate mechanism for accelerated neuroectodermal differentiation due to trisomy 21.; Authors - Kendra M Prutton
human,mouse,rat	WB	FASEB J; A crucial role for Nox 1 in redox-dependent regulation of Wnt-β-catenin signaling.; Authors - Kajla Seheli S
human	WB	Antioxidants (Basel); Enzymatic Depletion of Mitochondrial Inorganic Polyphosphate (polyP) Increases the Generation of Reactive Oxygen Species (ROS) and the Activity of the Pentose Phosphate Pathway (PPP) in Mammalian Cells.; Authors - Vedangi Hambardikar
human	WB,IF	Antioxidants (Basel); Nucleoredoxin Plays a Key Role in the Maintenance of Retinal Pigmented Epithelium Differentiation.; Authors - Mariana I Holubiec ; KD Validated
human	IHC	Cell Death Dis; NXN suppresses metastasis of hepatocellular carcinoma by promoting degradation of Snail through binding to DUB3.; Authors - Yuanping Zhang