Anticorps Monoclonal anti-NXN

NXN Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2C6A4

N° de cat : 66603-1-PBS

Synonymes

EC:1.8.1.8, NRX, Nucleoredoxin, TRG 4

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Galerie de données de validation

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  WB analysis using 66603-1-Ig (same clone as 66603-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66603-1-Ig (NXN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66603-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66603-1-Ig (same clone as 66603-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66603-1-Ig (NXN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66603-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66603-1-Ig (same clone as 66603-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66603-1-Ig (NXN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66603-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 66603-1-Ig (same clone as 66603-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using NXN antibody (66603-1-Ig, Clone: 2C6A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66603-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66603-1-PBS cible NXN dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NXN Protéine recombinante Ag9248
• Nom complet: nucleoredoxin
• Masse moléculaire calculée: 435 aa, 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC009327
• Symbole du gène: NXN
• Identification du gène (NCBI): 64359
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Nucleoredoxin (NRX/NXN), a relatively uncharacterized member of the TRX family protein, has recently been reported to regulate the Wnt/β-catenin pathway, which itself regulates cell fate and early development, in a redox-dependent manner(PMID:17567240). Northern blot analysis detected several nucleoredoxin splice variants in adult mouse tissues, with highest expression in testis and skin and lowest expression in liver(PMID:9119370). It has 3 isoforms produced by alternative splicing. This antibody is specific to NXN.

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