Anticorps Monoclonal anti-NXT1

NXT1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2C10A2

N° de cat : 67680-1-PBS

Synonymes

MTR2, NTF2 like export factor 1, NTF2 related export protein 1, NXT1, P15, Protein p15

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Galerie de données de validation

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  WB analysis using 67680-1-Ig (same clone as 67680-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67680-1-Ig (NXT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67680-1-PBS in a different storage buffer formulation.

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  WB analysis using 67680-1-Ig (same clone as 67680-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67680-1-Ig (NXT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67680-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67680-1-Ig (same clone as 67680-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NXT1 antibody (67680-1-Ig, Clone: 2C10A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67680-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67680-1-PBS cible NXT1 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NXT1 Protéine recombinante Ag30039
• Nom complet: NTF2-like export factor 1
• Masse moléculaire calculée: 15 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC000759
• Symbole du gène: NXT1
• Identification du gène (NCBI): 29107
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NXT1 is located in the nuclear envelope and is homologous to nuclear transport factor 2. NXT1 functions as a nuclear export factor in both RAN (Ras-related nuclear protein)- and CRM1 (required for chromosome region maintenance)-dependent pathways. It is found to stimulate the export of U1 snRNA in RAN- and CRM1-dependent pathways and the export of tRNA and mRNA in a CRM1-independent pathway. NXT1 also heterodimerizes with Tap protein and regulates the ability of Tap protein to mediate nuclear mRNA export.

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