Anticorps Recombinant de lapin anti-NCAM1/CD56

NCAM1/CD56 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240936B9

N° de cat : 83883-2-RR

Synonymes

Ncam1, CD56, 240936B9, NCAM 1, Ncam

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Galerie de données de validation

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  WB analysis of C6 using 83883-2-RR

  C6 cells were subjected to SDS PAGE followed by western blot with 83883-2-RR (Ncam1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 83883-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83883-2-RR (NCAM1/CD56 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83883-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83883-2-RR against Mouse Ncam1 were performed. The affinity constant is 0.833 nM.

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Applications testées

• Résultats positifs en WB: tissu de cervelet de souris, cellules C6, tissu cérébral de rat, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83883-2-RR cible NCAM1/CD56 dans les applications de WB, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: neural cell adhesion molecule 1
• Masse moléculaire calculée: 119 kDa
• Poids moléculaire observé: 120 kDa, 140 kDa, 180 kDa
• Symbole du gène: Ncam1
• Identification du gène (NCBI): 17967
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells, and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by the addition of polysialic acid (PSA) (PMID: 14976519). Several other isoforms have also been described (PMID: 1856291).

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