Anticorps Recombinant de lapin anti-NCAM1/CD56

NCAM1/CD56 Recombinant Antibody for IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240936B10

N° de cat : 83883-5-PBS

Synonymes

CD56, Ncam1, 240936B10, E NCAM, Ncam

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Galerie de données de validation

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  IHC staining of mouse brain using 83883-5-RR (same clone as 83883-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83883-5-RR (NCAM1/CD56 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83883-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 83883-5-RR (same clone as 83883-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83883-5-RR (NCAM1/CD56 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83883-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 83883-5-RR (same clone as 83883-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83883-5-RR (NCAM1/CD56 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83883-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 83883-5-RR (same clone as 83883-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83883-5-RR (NCAM1/CD56 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83883-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 83883-5-RR (same clone as 83883-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83883-5-RR (NCAM1/CD56 antibody) at dilution of 1:9000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83883-5-PBS in a different storage buffer formulation.

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  IF Staining of Neuro-2a using 83883-5-RR (same clone as 83883-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using Ncam1 antibody (83883-5-RR, Clone: 240936B10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83883-5-PBS in a different storage buffer formulation.

• 

  IF Staining of Neuro-2a using 83883-5-RR (same clone as 83883-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using Ncam1 antibody (83883-5-RR, Clone: 240936B10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83883-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83883-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83883-5-RR against Mouse Ncam1 were performed. The affinity constant is 0.184 nM.

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Informations sur le produit

83883-5-PBS cible NCAM1/CD56 dans les applications de IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NCAM1/CD56 Protéine recombinante Eg0652
• Nom complet: neural cell adhesion molecule 1
• Masse moléculaire calculée: 119 kDa
• Poids moléculaire observé: 120 kDa, 140 kDa, 180 kDa
• Symbole du gène: Ncam1
• Identification du gène (NCBI): 17967
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells, and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by the addition of polysialic acid (PSA) (PMID: 14976519). Several other isoforms have also been described (PMID: 1856291).

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