Anticorps Recombinant de lapin anti-Nectin-2/CD112

Nectin-2/CD112 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

241798A11

N° de cat : 84439-6-RR

Synonymes

241798A11, CD112, Herpes virus entry mediator B, HveB, mHveB

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Galerie de données de validation

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  WB analysis using 84439-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84439-6-RR (Nectin-2/CD112 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of RAW 264.7 using 84439-6-RR

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using Nectin-2/CD112 antibody (84439-6-RR, Clone: 241798A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7, cellules C2C12, cellules NIH/3T3, tissu testiculaire de souris
• Résultats positifs en IF/ICC: cellules RAW 264.7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84439-6-RR cible Nectin-2/CD112 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Nectin-2/CD112 Protéine recombinante Eg2019
• Nom complet: poliovirus receptor-related 2
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 65-75 kDa
• Numéro d’acquisition GenBank: NM_008990.3
• Symbole du gène: Nectin-2/CD112
• Identification du gène (NCBI): 19294
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nectin 2, also named as PVRL2, CD112, HVEB, PRR2 and PVRR2, is an adhesion molecule widely expressed in cell lines of different lineages, including hematopoietic, neuronal, endothelial and epithelial cells. CD112 belongs to a new family of immunoglobulin-like molecules that includes four members (CD111, CD112, PRR3 and CD155) sharing an ectodomain made of three Ig domains, of V and C types. CD112 is expressed in the myelo-monocytic and megakaryocytic hematopoietic lineages and the function in hematopoiesis is currently unknown. CD112 is an intercellular homophilic adhesion. CD112 localizes specifically at adherents junctions via its cytoplasmic interaction with the scaffold F-actin binding protein afadin. Disruption of the murine CD112 gene leads to infertility of male mice with morphologically aberrant spermatozoa. CD112 mediates entry of some alphaherpesvirus mutants (also named HveB) via its V domain. CD112 is involved in cell to cell spreading of viruses.

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