Anticorps Recombinant de lapin anti-Nectin-2/CD112

• Phare
• Validé par KD/KO

Nectin-2/CD112 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

241725D3

N° de cat : 84460-5-RR

Synonymes

Nectin-2, 241725D3, CD112, Herpes virus entry mediator B, Herpesvirus entry mediator B

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Galerie de données de validation

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  WB analysis of HAP1 using 84460-5-RR

  WB result of Nectin-2/CD112 antibody (84460-5-RR; 1:2000; room temperature for 1.5 hours) with wild-type and Nectin-2/CD112 knockout HAP1 cells.

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  WB analysis using 84460-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84460-5-RR (Nectin-2/CD112 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of K-562 using 84460-5-RR

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using Nectin-2/CD112 antibody (84460-5-RR, Clone: 241725D3 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84460-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84460-5-RR against Human Nectin-2/CD112 were performed. The affinity constant is 0.314 nM.

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Applications testées

• Résultats positifs en WB: cellules HT-29, cellules A549, cellules MDA-MB-231
• Résultats positifs en IF/ICC: cellules K-562,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84460-5-RR cible Nectin-2/CD112 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Nectin-2/CD112 Protéine recombinante Eg1670
• Nom complet: poliovirus receptor-related 2 (herpesvirus entry mediator B)
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 65-75 kDa
• Numéro d’acquisition GenBank: NM_001042724.2
• Symbole du gène: Nectin-2/CD112
• Identification du gène (NCBI): 5819
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nectin 2, also named as PVRL2, CD112, HVEB, PRR2 and PVRR2, is an adhesion molecule widely expressed in cell lines of different lineages, including hematopoietic, neuronal, endothelial and epithelial cells. CD112 belongs to a new family of immunoglobulin-like molecules that includes four members (CD111, CD112, PRR3 and CD155) sharing an ectodomain made of three Ig domains, of V and C types. CD112 is expressed in the myelo-monocytic and megakaryocytic hematopoietic lineages and the function in hematopoiesis is currently unknown. CD112 is an intercellular homophilic adhesion. CD112 localizes specifically at adherents junctions via its cytoplasmic interaction with the scaffold F-actin binding protein afadin. Disruption of the murine CD112 gene leads to infertility of male mice with morphologically aberrant spermatozoa. CD112 mediates entry of some alphaherpesvirus mutants (also named HveB) via its V domain. CD112 is involved in cell to cell spreading of viruses.

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