Anticorps Recombinant de lapin anti-Nectin-2/CD112

Nectin-2/CD112 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

241798A11

N° de cat : 98231-1-RR

Synonymes

241798A11, CD112, Herpes virus entry mediator B, HveB, mHveB

---

Galerie de données de validation

• 

  FC experiment of NIH/3T3 using 98231-1-RR

  1x10^6 NIH/3T3 cells were surface stained with 0.25 ug Anti-Mouse Nectin-2/CD112 Rabbit Recombinant Antibody (98231-1-RR, Clone: 241798A11)(red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  FC experiment of NIH/3T3 using 98231-1-RR

  1x10^6 NIH/3T3 cells were surface stained with 0.25 ug Anti-Mouse Nectin-2/CD112 Rabbit Recombinant Antibody (98231-1-RR, Clone: 241798A11)(red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98231-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98231-1-RR against Mouse Nectin-2/CD112 were performed. The affinity constant is below 1 pM.

---

Applications testées

• Résultats positifs en cytométrie: cellules NIH/3T3,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98231-1-RR cible Nectin-2/CD112 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Nectin-2/CD112 Protéine recombinante Eg2019
• Nom complet: poliovirus receptor-related 2
• Masse moléculaire calculée: 57 kDa
• Numéro d’acquisition GenBank: NM_008990.3
• Symbole du gène: Nectin-2/CD112
• Identification du gène (NCBI): 19294
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

Nectin 2, also named as PVRL2, CD112, HVEB, PRR2 and PVRR2, is an adhesion molecule widely expressed in cell lines of different lineages, including hematopoietic, neuronal, endothelial and epithelial cells. CD112 belongs to a new family of immunoglobulin-like molecules that includes four members (CD111, CD112, PRR3 and CD155) sharing an ectodomain made of three Ig domains, of V and C types. CD112 is expressed in the myelo-monocytic and megakaryocytic hematopoietic lineages and the function in hematopoiesis is currently unknown. CD112 is an intercellular homophilic adhesion. CD112 localizes specifically at adherents junctions via its cytoplasmic interaction with the scaffold F-actin binding protein afadin. Disruption of the murine CD112 gene leads to infertility of male mice with morphologically aberrant spermatozoa. CD112 mediates entry of some alphaherpesvirus mutants (also named HveB) via its V domain. CD112 is involved in cell to cell spreading of viruses.

{{ptg:RelatedPrimaryAntibodies}}