Anticorps Polyclonal de lapin anti-Nephrin

Nephrin Polyclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 22912-1-AP

Synonymes

NPHS1, CNF, NPHN, NPHS 1, Renal glomerulus-specific cell adhesion receptor

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Galerie de données de validation

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  WB analysis using 22912-1-AP

  Mouse kidney tissues were subjected to SDS PAGE followed by western blot with 22912-1-AP (Nephrin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 22912-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22912-1-AP (Nephrin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 22912-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22912-1-AP (Nephrin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 22912-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22912-1-AP (Nephrin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 22912-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22912-1-AP (Nephrin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 22912-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 22912-1-AP (Nephrin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat kidney using 22912-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat kidney tissue using Nephrin antibody (22912-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris,
• Résultats positifs en IHC: tissu rénal de souris, tissu rénal de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu rénal de rat,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

22912-1-AP cible Nephrin dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Nephrin Protéine recombinante Ag18994
• Nom complet: nephrosis 1, congenital, Finnish type (nephrin)
• Masse moléculaire calculée: 1241 aa, 135 kDa
• Poids moléculaire observé: 120-130 kDa
• Numéro d’acquisition GenBank: BC156935
• Symbole du gène: Nephrin
• Identification du gène (NCBI): 4868
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Podocytes are a major component of the glomerular blood filtration barrier. Podocyte protein Nephrin, also known as NPHS1, is the first to be identified as a central component of the slit diaphragm (SD), which is the podocyte cell-cell junction. Mutations in Nephrin lead to congenital nephrosis, such as steroid-resistant proteinuria and congenital nephrotic syndrome of the Finnish type (CNF).

Publications

Species	Application	Title
mouse	WB	BMC Med Genomics; Deciphering the molecular nexus of BTG2 in periodontitis and diabetic kidney disease; Authors - Binhui Pan
human	IHC	J Nephrol; Association of the podocyte phenotype with extracapillary hypercellularity in patients with diabetic kidney disease; Authors - Weixia Han
rat	IF	J Ethnopharmacol; Supplemented Gegen Qinlian Decoction Formula attenuates podocyte mitochondrial fission and renal fibrosis in diabetic kidney disease by inhibiting TNF-α-mediated necroptosis, compared with empagliflozin; Authors - Yu Wang
mouse	WB	Antioxidants (Basel); Identification and Validation of the miR/RAS/RUNX2 Autophagy Regulatory Network in AngII-Induced Hypertensive Nephropathy in MPC5 Cells Treated with Hydrogen Sulfide Donors; Authors - Qing Ye
mouse	IF	Exp Cell Res; Fbxo11 maintains mitochondrial function and prevents podocyte injury in adriamycin-induced nephropathy by mediating the ubiquitin degradation of Fosl2; Authors - Yanhua Jin
rat	WB,IHC	Mitochondrion; The compound XueShuanTong promotes podocyte mitochondrial autophagy via the AMPK/mTOR pathway to alleviate diabetic nephropathy injury; Authors - Chuangbiao Zhang

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